Lee, Chia Hau, et al. “Antioxidant and antidiabetic properties of bioactive peptides from soursop (Annona muricata) leaf biomass.” Biomass Conversion and Biorefinery (2023): 1-14. https://doi.org/10.1007/s13399-023-03993-5

Abstract

This study investigated the antioxidant and antidiabetic properties of soursop (Annona muricata) leaf biomass hydrolysates (SLH) extracted from the leaf proteins. The results revealed that flavourzyme-generated SLH showed the highest peptide content when compared to alcalase- and trypsin-generated SLH. Application of response surface methodology (RSM) remarkably increased peptide content under the optimized conditions at an E/S ratio of 4% (w/w), hydrolysis time of 4 h, a temperature of 50 °C, and pH of 7. SLH demonstrated stronger antioxidant (DPPH (22.92%) and ABTS (90.55%)) and antidiabetic (α-glucosidase inhibition (66.44%) and α-amylase inhibition (58.50%)) activities. A total of 14 peptides were identified as bioactive peptides, among which two peptides (FLAISPKAMK and WDSKWTHI) possessed the highest binding probability toward α-glucosidase and α-amylase. These peptides contain hydrophobic amino acids, which are important in improving the antioxidant activities of peptides. In conclusion, SLH could be useful for diabetes prevention.