Yi Wang et al, Antihypertensive effects in vitro and vivo of novel angiotensin-converting enzyme inhibitory peptides from bovine bone gelatin hydrolysate. 12/19/2019

In this study, we investigated the antihypertensive effects in vitro and vivo of novel angiotensin-converting enzyme inhibitory (ACEI) peptides purified and identified from bovine bone gelatin hydrolysate (BGH). Thirteen ACEI peptides were identified from BGH, among which RGL-(Hyp)-GL and RGM-(Hyp)-GF exhibited high ACE inhibition with IC50 values of 1.44 µM and 10.23 µM. Molecular docking predicted that RGM-(Hyp)-GF and the ACE residues of Glu384, His513 and Lys511 formed hydrogen-bonding interactions at distances of 2.57, 2.99 and 2.42+3.0 Å. RGL-(Hyp)-GL formed hydrogen bonds with Lys511 and Tyr523 and generated hydrogen-bonding interactions with His387 and Glu411 in the zinc(II) complexation motif at distances of 2.74 and 3.03+1.93 Å. The maximal decrements in systolic blood pressure (SBP) in spontaneously hypertensive rats (SHRs) induced by one-time gavage of RGL and RGM at 30 mg/kg were 31.3 and 38.6 mmHg. RGL-(Hyp)-GL had higher enzyme degradation resistance than RGM-(Hyp)-GF in vitro incubation in rat plasma, and they were sequentially degraded into pentapeptides and tetrapeptides within 2 hours. Our results indicate that BGH can serve as a nutritional candidate to control blood pressure.