Translation events that were previously thought to result in non-biologically relevant proteins are now being reconsidered. Increasing evidence shows that some non-canonical proteins may play an important role in immunity and disease. This paper examines the unique features of the non-canonical translatome and how they may influence tumor immunosurveillance.
Suprabasin-derived bioactive peptides identified by plasma peptidomics
Isolating and identifying native peptides in serum samples can be very difficult by LC-MS/MS data in plasma studies. Taguchi and colleagues address these challenges and show that this approach to plasma peptidomics can ID unknown low abundance peptides with biological activity.
Proteogenomic Analysis Unveils the HLA Class I-Presented Immunopeptidome in Melanoma and EGFR-Mutant Lung Adenocarcinoma
One of the hurdles in the development of immunopeptidomic tools is the heterogeneity of the tumour-associated signals resulting in challenges with traditional database search strategies for MS data. Here Qi et al. from NIH are working to remove this hurdle by combining de novo peptide antigen sequencing and custom database strategies.
Human Blood and Bird Egg Proteins Identified in Red Paint Covering a 1000-Year-Old Gold Mask from Peru
In a recent study by Pires et al., LC-MS/MS was used to study the protein components of the binding material in a paint sample that remained on a 1000-Year-Old Gold Mask, presumably by members of the Sicán culture of Peru.
RHybridFinder: An R package to process immunopeptidomic data for putative hybrid peptide discovery
Immunopeptidomics is the study of the processed peptides by LC-MS/MS and serves as a tool for the identification of novel antigens. This paper by Saab and others presents a novel statistical computing method called RHybridFinder (RHF).
An Integrated Strategy for Mass Spectrometry-Based Multiomics Analysis of Single Cells
Looking to optimize methods used for single cell proteomics from sample capture, to peptide extraction, through LC-MS/MS & data analysis? Li et al. recently published a workflow for integrated proteome, phosphoproteome, & metabolome analysis from a single cell that addresses many single cell proteomics challenges.
Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome
In a recent study by Ho et al, LC-MS/MS was used to examine the influence of a rocaglate on the cellular proteome & translatome to study the effects of these drugs on the process of protein production. Learn more & how translational remodeling effects drug’s potency
Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides
Using 4D proteomics, Realis-Doyelle, E., et al. were able to identify 25 neuropeptide precursors involved in the regulation of reproduction in Pacific oysters. By using PEAKS IMS, the researchers were able to take advantage of the 4th-dimension of data generated by the timsTOF Pro.
Murine neuronatin deficiency is associated with a hypervariable food intake and bimodal obesity
To study how a change in genetic information, like neuronatin deficiency, has on food intake and body mass, Ciminoet et al. used LC-MS/MS to study the peptides of pancreatic hormones in Nnat deficient and wildtype mice. Learn about this study and how this approach can be used for other studies, like hormone processing, receptor sensitivity, etc.
Epigenetic inactivation of the autophagy–lysosomal system in appendix in Parkinson’s disease
The link between gut & brain health is a target of interest for Parkinson’s disease (PD) research. In this paper, Gordevicius et al, use multi-molecular methods to study PD pathology from epigenetic changes in DNA & altered transcription, to the resulting modified protein abundance.