One of the hurdles in the development of immunopeptidomic tools is the heterogeneity of the tumour-associated signals resulting in challenges with traditional database search strategies for MS data. Here Qi et al. from NIH are working to remove this hurdle by combining de novo peptide antigen sequencing and custom database strategies.
Human Blood and Bird Egg Proteins Identified in Red Paint Covering a 1000-Year-Old Gold Mask from Peru
In a recent study by Pires et al., LC-MS/MS was used to study the protein components of the binding material in a paint sample that remained on a 1000-Year-Old Gold Mask, presumably by members of the Sicán culture of Peru.
RHybridFinder: An R package to process immunopeptidomic data for putative hybrid peptide discovery
Immunopeptidomics is the study of the processed peptides by LC-MS/MS and serves as a tool for the identification of novel antigens. This paper by Saab and others presents a novel statistical computing method called RHybridFinder (RHF).
An Integrated Strategy for Mass Spectrometry-Based Multiomics Analysis of Single Cells
Looking to optimize methods used for single cell proteomics from sample capture, to peptide extraction, through LC-MS/MS & data analysis? Li et al. recently published a workflow for integrated proteome, phosphoproteome, & metabolome analysis from a single cell that addresses many single cell proteomics challenges.
Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome
In a recent study by Ho et al, LC-MS/MS was used to examine the influence of a rocaglate on the cellular proteome & translatome to study the effects of these drugs on the process of protein production. Learn more & how translational remodeling effects drug’s potency
Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides
Using 4D proteomics, Realis-Doyelle, E., et al. were able to identify 25 neuropeptide precursors involved in the regulation of reproduction in Pacific oysters. By using PEAKS IMS, the researchers were able to take advantage of the 4th-dimension of data generated by the timsTOF Pro.
Murine neuronatin deficiency is associated with a hypervariable food intake and bimodal obesity
To study how a change in genetic information, like neuronatin deficiency, has on food intake and body mass, Ciminoet et al. used LC-MS/MS to study the peptides of pancreatic hormones in Nnat deficient and wildtype mice. Learn about this study and how this approach can be used for other studies, like hormone processing, receptor sensitivity, etc.
Epigenetic inactivation of the autophagy–lysosomal system in appendix in Parkinson’s disease
The link between gut & brain health is a target of interest for Parkinson’s disease (PD) research. In this paper, Gordevicius et al, use multi-molecular methods to study PD pathology from epigenetic changes in DNA & altered transcription, to the resulting modified protein abundance.
In vitro metabolism of synthetic Elabela/Toddler (ELA-32) peptide in human plasma and kidney homogenates analyzed with mass spectrometry and validation of endogenous peptide quantification in tissues by ELISA
Studies about cardiovascular diseases have shown the Elabela/Toddler (ELA) ligand to be a beneficial apelin Rc. agonist. In a recent paper, Nyimanu et al. used LC-MS/MS methods to examine how the different forms of ELA individually impact signaling networks in the human body.
A complete workflow for discovering small bioactive peptides in foods by LC-MS/MS: A case study on almonds
In our food and supplements, we see new functional foods and nutraceuticals with new claims everyday. But do these compounds show any bioactivity to support these claims? Huang et al show Mass spec can be a great tool to study these compound.