Proteomics examines the diverse array of proteins expressed in a cell, tissue, or organism under specific biological conditions, offering crucial insights into cellular function, disease mechanisms, and therapeutic targets. By analysing protein expression, modifications, and interactions, proteomics can reveal changes in cell state, response to stimuli, or disease progression. Beyond cellular studies, proteomics is also essential for the characterisation of purified proteins, enabling the identification of sequence variants, post-translational modifications (PTMs), and structural properties that impact function and stability. These insights are invaluable for biomedical research, drug development, and biotechnology applications.
Advanced biomarker discovery for diseases such as cancer and neurodegenerative disorders.
Understanding protein pathways can uncover molecular mechanisms of diseases, elucidating drug targets.
Identifying proteome-wide changes caused by environmental, agricultural and health related conditions.
Identifications of patient specific antigen profiles for the development of immunotherapies and cancer vaccines.
Studying proteomes from different ecosystems to understand biodiversity, adaptation, and ecological processes.
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Protein Identification and PTM Analysis
Service Description:
We process in-solution or in-gel samples and offer customised digestion with 5 different enzyme options available to enhance protein coverage. Liquid chromatography tandem mass spectrometry (LC-MS/MS) is used to generate raw data. Our latest PEAKS Studio software is used for both de novo sequencing and database searching to provide sensitive and accurate peptide/protein identification results. A targeted search for any post-translational modifications (PTMs) in PEAKS Studio can be included as an optional add-on service. Our user-friendly PEAKS Studio Viewer (included) provides an easy way to further investigate your data and extract images of the results.
Workflow:
In-solution/in-gel digestion → LC-MS/MS → Data analysis
What we need:
- 20μg of sample provided in-solution or as gel bands
- Samples must be free of detergents and polymers (Triton X-100, Tween-20, PEG, etc.)
- Buffer information for in-solution samples
What we deliver:
- PEAKS Studio project file containing database search results and PTM analysis
- PEAKS Studio Viewer
- Raw data files
Want to know more? Request more details to learn why our methodology and experience gives you better results than our competitors. Have questions? We’ll be happy to answer any of your inquiries today.