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What we will provide

  • Comprehensive training of PEAKS software
  • PEAKS Software Training License
  • Training Certificate
  • Morning & Afternoon Breaks
  • Catered Lunch

What you should bring

  • Be sure to bring your laptop (Microsoft Windows, 4GB RAM)

What we will be talking about

 

1. Protein/peptide identification with PEAKS

  • Database search
  • De novo sequencing
  • Homology search
  • Case Study 1 – Identification of endogenous peptides
  • Case Study 2 – In-depth proteomics analysis for PTMs and sequence variants

 

2. Protein/peptide quantification with PEAKS

  • Label-free quantification (new for 2017)
  • Labelling (TMT, iTRAQ, SILAC) quantification
  • MultiNotch MS3 quantification
  • Case study 3 – label-free quantification for biomarker discovery
  • Case study 4 – SILAC to identify kinase signaling network at peptide level

 

3. Protein characterization with PEAKS AB (new as of 2016)

  • Protein de novo sequencing with LC-MS/MS
  • Quantitative analysis of protein isoforms
  • Therapeutic protein sequence validation
  • Case study 5 – Antibody protein sequencing with multiple-enzyme digests
  • Case study 6 – Analytical characterization of Biosimilar products by LC-MS/MS