May 24-25, 2017
Location:
Lady Margaret Hall
University of Oxford
Norham Gardens, Oxford
Time: 9:30am – 4:30pm
Cost: £300 GBP
Registration – Closed
What we will provide
- Comprehensive training of PEAKS software
- PEAKS Software Training License
- Training Certificate
- Morning & Afternoon Breaks
- Catered Lunch
What you should bring
- Be sure to bring your laptop (Microsoft Windows, 4GB RAM)
What we will be talking about
1. Protein/peptide identification with PEAKS
- Database search
- De novo sequencing
- Homology search
- Case Study 1 – Identification of endogenous peptides
- Case Study 2 – In-depth proteomics analysis for PTMs and sequence variants
2. Protein/peptide quantification with PEAKS
- Label-free quantification (new for 2017)
- Labelling (TMT, iTRAQ, SILAC) quantification
- MultiNotch MS3 quantification
- Case study 3 – label-free quantification for biomarker discovery
- Case study 4 – SILAC to identify kinase signaling network at peptide level
3. Protein characterization with PEAKS AB (new as of 2016)
- Protein de novo sequencing with LC-MS/MS
- Quantitative analysis of protein isoforms
- Therapeutic protein sequence validation
- Case study 5 – Antibody protein sequencing with multiple-enzyme digests
- Case study 6 – Analytical characterization of Biosimilar products by LC-MS/MS