Authors:
Natalie Korkola, PhD, and Kristina Jurcic MSc

Protein glycosylation is one of the most common post translational modifications (PTMs). It plays a crucial
role in many biological processes and changes in glycosylation can signal disease. Thus, studying protein
glycosylation is important for our understanding of the proteome and for discovering potential drug targets. However, due to glycan heterogeneity and complexity of data, glycosylation is challenging to study using mass spectrometry methods. Specifically, the main challenges come from diverse glycan structures, which vary from mono to complex oligosaccharides, as well as the existence of multiple glycans per peptides and proteins. In addition, mass spectra are complicated by the presence of both peptide backbone and glycan fragments. PEAKS GlycanFinder provides a unique approach to tackle these challenges and offers an accurate and sensitive comprehensive solution for qualitative and quantitative glycoproteomics and glycomics.